rna pretreatment kit Search Results


ultrapure rna kit  (Beijing CWBio)
90
Beijing CWBio ultrapure rna kit
Ultrapure Rna Kit, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/bio_rxiv__2022__03__15__484441-158-10-13?v=Beijing+CWBio
Average 90 stars, based on 1 article reviews
ultrapure rna kit - by Bioz Stars, 2026-08
90/100 stars
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rtstar trna pretreatment and first-strand cdna synthesis kit as-fs-004  (Arraystar inc)
90
Arraystar inc rtstar trna pretreatment and first-strand cdna synthesis kit as-fs-004
Rtstar Trna Pretreatment And First Strand Cdna Synthesis Kit As Fs 004, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pm38849541-272-17-29?v=Arraystar+inc
Average 90 stars, based on 1 article reviews
rtstar trna pretreatment and first-strand cdna synthesis kit as-fs-004 - by Bioz Stars, 2026-08
90/100 stars
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illustra rnaspin mini rna isolation kit  (Cytiva Europe)
93
Cytiva Europe illustra rnaspin mini rna isolation kit
Illustra Rnaspin Mini Rna Isolation Kit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pmc05927545-387-22-28?v=Cytiva+Europe
Average 93 stars, based on 1 article reviews
illustra rnaspin mini rna isolation kit - by Bioz Stars, 2026-08
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rna pretreatment kit #as-fs-005  (Arraystar inc)
90
Arraystar inc rna pretreatment kit #as-fs-005
Rna Pretreatment Kit #As Fs 005, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pm33588913-66-10-15?v=Arraystar+inc
Average 90 stars, based on 1 article reviews
rna pretreatment kit #as-fs-005 - by Bioz Stars, 2026-08
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sirna pretreatment  (Miltenyi Biotec)
95
Miltenyi Biotec sirna pretreatment
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Sirna Pretreatment, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pmc08409991-243-24-40?v=Miltenyi+Biotec
Average 95 stars, based on 1 article reviews
sirna pretreatment - by Bioz Stars, 2026-08
95/100 stars
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qiaamp viral rna mini kit  (Qiagen)
99
Qiagen qiaamp viral rna mini kit
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Qiaamp Viral Rna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/10__1186_slash_1743___422x___11___157-120-11-16?v=Qiagen
Average 99 stars, based on 1 article reviews
qiaamp viral rna mini kit - by Bioz Stars, 2026-08
99/100 stars
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rnase free dnasei  (Thermo Fisher)
99
Thermo Fisher rnase free dnasei
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Rnase Free Dnasei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pm22765286-201-29-31?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
rnase free dnasei - by Bioz Stars, 2026-08
99/100 stars
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fastpure cell tissue total rna isolation kit  (Vazyme Biotech Co)
99
Vazyme Biotech Co fastpure cell tissue total rna isolation kit
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Fastpure Cell Tissue Total Rna Isolation Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pmc10070975-39-8-18?v=Vazyme+Biotech+Co
Average 99 stars, based on 1 article reviews
fastpure cell tissue total rna isolation kit - by Bioz Stars, 2026-08
99/100 stars
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invitrogen turbo dna free kit  (Thermo Fisher)
99
Thermo Fisher invitrogen turbo dna free kit
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Invitrogen Turbo Dna Free Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pmc06789923__pnas__1902747116__sapp-40-5-5?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
invitrogen turbo dna free kit - by Bioz Stars, 2026-08
99/100 stars
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nebnext ultra rna library prep kit for illumina  (Illumina Inc)
96
Illumina Inc nebnext ultra rna library prep kit for illumina
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Nebnext Ultra Rna Library Prep Kit For Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pmc09666723-55-11-18?v=Illumina+Inc
Average 96 stars, based on 1 article reviews
nebnext ultra rna library prep kit for illumina - by Bioz Stars, 2026-08
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rna extraction kit  (Promega)
90
Promega rna extraction kit
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Rna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pmc09842942-187-12-15?v=Promega
Average 90 stars, based on 1 article reviews
rna extraction kit - by Bioz Stars, 2026-08
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viral rna extraction kit  (Eiken Chemical)
90
Eiken Chemical viral rna extraction kit
CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a <t>siRNA</t> or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Viral Rna Extraction Kit, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+pretreatment+kit/pm33865897-610-13-17?v=Eiken+Chemical
Average 90 stars, based on 1 article reviews
viral rna extraction kit - by Bioz Stars, 2026-08
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Image Search Results


CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a siRNA or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: JCI Insight

Article Title: PLA2G4A promotes right-sided colorectal cancer progression by inducing CD39+γδ Treg polarization

doi: 10.1172/jci.insight.148028

Figure Lengend Snippet: CT26- Pla2g4a and CT26-Vec cells were cocultured with intraepithelial lymphocytes derived from the ileum (IL-IELs), colon (CO-IELs), or spleen of BALB/c mice. (A and C ) Representative pictures showed the number of CT26 cells in the coculture system after 12 ( A ) and 36 hours ( C ). Scale bars: 200 μm. ( B and D ) Bar diagrams summarizes the cell numbers of CT26- Pla2g4a and CT26-Vec cells cocultured with IL-IELs after 12 ( B ) and 36 ( D ) hours. Data are shown as mean ± SEM, using 2-way ANOVA followed by Sidak’s multiple-comparisons test. ( E–G ) Representative histogram plot and bar diagram summarizes the expression of CD39 on γδ T cells in IL-IELs ( E ), CO-IELs ( F ) and splenocytes ( G ) group. ( H ) Representative flow cytometric analysis of CD39 + Tregs in the total CD3 + T cells from splenocytes. Bar diagram summarizes the percentages of CD39 + γδ Tregs. Unpaired 2-tailed t test was used to compare variables. ( I ) Silencing Pla2g4a in CT26- Pla2g4a rescued the antitumor response mediated by γδ T cells. CT26- Pla2g4a were transfected with Pla2g4a siRNA or control siRNA. After 24 hours, CT26-Vec, CT26- Pla2g4a , and CT26- Pla2g4a –knock-down cells were cocultured with γδ T cells sorted from BALB/c mice with or without anti-PLA2G4A antibody (5 mg/mL). Scale bars: 200 μm. ( J ) Bar diagram summarizes the cell number of CT26. ( K ) Representative histogram plot of CD39 expression on γδ T cells in each group by flow cytometry. Bar diagram summarizes the frequency of CD39 + γδ Tregs/γδ T cells. One-way ANOVA followed by Tukey’s multiple-comparison test was used for multiple-group analysis. All of the experiments were repeated 3 times; n = 3/group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: For rescue experiments, γδ T cells sorted from IELs of BALB/c mice were cocultured with CT26-Vec or CT26- Pla2g4a (γδ T/CT26 = 1:1) with siRNA pretreatment for 2 hours using a mouse TCR γ/δ T cell Isolation Kit (catalog 130-092-125; Miltenyi Biotec).

Techniques: Derivative Assay, Expressing, Transfection, Control, Knockdown, Flow Cytometry, Comparison